Francisco de Peñalosa (c. 1470 – April 1, 1528) was a Spanish composer of the middle Renaissance.
He was born in Talavera de la Reina in the province of Toledo. He spent most of his career in Seville, serving as the maestro di capilla, though he also spent time in Burgos, and three years in Rome at the papal chapel (1518–1521). He died in Seville.Peñalosa was one of the most famous Spanish composers of the generation before Cristóbal de Morales, and his compositions were highly regarded at the time. Unfortunately for him, his music was not widely distributed; he did not benefit from the invention of printing, since he mostly remained in Spain, away from cities such as Venice and Antwerp which were the first centers of printed music. Later generations of Spanish composers—Guerrero, Morales, Victoria—went to Italy for parts of their careers, where their compositions were printed and were as widely distributed as the music of the Franco-Flemish composers who dominated music in Europe in the 16th century.